• DNA Metabarcoding of Preservative Ethanol Reveals Changes in Invertebrate Community Composition Following Rotenone Treatment 

      Kjærstad, Gaute; Majaneva, Markus; Falahati-Anbaran, Mohsen; Topstad, Lasse; Finstad, Anders Gravbrøt; Arnekleiv, Jo Vegar; Ekrem, Torbjørn (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-06-01)
      The botanical compound rotenone is extensively used to eradicate populations and reduce the negative impacts of freshwater invasive animals. The method is controversial as non-target organisms often are negatively affected, but these effects are highly variable among taxa and may be difficult to monitor on species-level as many invertebrates are challenging or costly to identify using morphology. ...
    • Multi-marker DNA metabarcoding reflects tardigrade diversity in different habitats 

      Topstad, Lasse; Guidetti, Roberto; Majaneva, Markus; Ekrem, Torbjørn (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-11-03)
      Like meiofauna in general, tardigrades are often neglected in ecological and environmental surveys. Tardigrades occur in all parts of the world, from deep marine sediments to alpine environments, and are present in most ecosystems. They are therefore potentially good candidates for biomonitoring programs. However, sampling of these minute animals is both tedious and time-consuming, impeding their ...
    • Sedimentary ancient DNA reveals local vegetation changes driven by glacial activity and climate. 

      Elliott, Lucas Dane; Rijal, Dilli Prasad; Brown, Antony; Bakke, Jostein; Topstad, Lasse; Heintzman, Peter D.; Alsos, Inger Greve (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-01-07)
      Disentangling the effects of glaciers and climate on vegetation is complicated by the confounding role that climate plays in both systems. We reconstructed changes in vegetation occurring over the Holocene at Jøkelvatnet, a lake located directly downstream from the Langfjordjøkel glacier in northern Norway. We used a sedimentary ancient DNA (sedaDNA) metabarcoding dataset of 38 samples from a ...
    • Tardigrade diversity and community composition across Norwegian boreal forests 

      Guidetti, Roberto; Jönsson, K. Ingemar; Kaczmarek, Łukasz; Meier, Terje; Speed, James David Mervyn; Prestø, Tommy; Stur, Elisabeth; Topstad, Lasse; Cesari, Michele; Roszkowska, Milena; Zawierucha, Krzysztof; Hassel, Kristian; Ekrem, Torbjørn (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-10-26)
      Tardigrades are common in many terrestrial environments and habitats. Although little is known on their ecological preferences, previous studies found diversity and community composition significantly affected by various variables. This study associated tardigrade species’ records with climatic variables, forest type, and substrate categories exploring tardigrade diversity and species communities ...
    • An urban Blitz with a twist: rapid biodiversity assessment using aquatic environmental DNA 

      Hupało, Kamil; Majaneva, Markus; Czachur, Molly Victoria; Sire, Lucas; Marquina, Daniel; Lijtmaer, Darío A.; Ivanov, Vladislav; Leidenberger, Sonja; Čiampor, Fedor; Čiamporová-Zat'ovičová, Zuzana; Mendes, Izabela S.; Desiderato, Andrea; Topstad, Lasse; Meganck, Kenny; Hariz Z. A., Danial; Kjærstad, Gaute; Lin, Xiao-Long; Price, Benjamin; Stevens, Mark; Ekrem, Torbjørn; Deiner, Kristy (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-10-24)
      As global biodiversity declines, there is an increasing need to create an educated and engaged society. Having people of all ages participate in measuring biodiversity where they live helps to create awareness. Recently, the use of environmental DNA (eDNA) for biodiversity surveys has gained momentum. Here, we explore whether sampling eDNA and sequencing it can be used as a means of rapidly surveying ...